3D Genomics: A New Dimension in DNA​
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DNA is organized within the nucleus into a complex but highly deliberate, three-dimensional structure that plays a critical role in gene regulation and other nuclear processes. Hi-C technique utilizes NGS to generate a comprehensive map of genome-wide chromatin interactions. It has been used in a wide range of studies, including the organization of functional genomic elements such as enhancers and promoters and their associated protein factors, the mapping of long-range chromatin interactions, and the study of epigenetic regulation of gene expression.
WHAT HI-C REVEALS
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Gene Regulation — Map long-range enhancer–promoter interactions that control transcriptional activity.
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Chromatin Architecture — Resolve topological features like loops, TADs and A/B compartments.
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Disease Mechanisms — Understand how altered 3D genome structures contribute to cancer, developmental disorders, and other diseases.
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Cell Identity — Characterize 3D genome conformations unique to cell types, states, and lineages.
Hi-C WORKFLOW
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1. Experimental Design
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2. Sample Preparation
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3. Library Preparation
4. Sequencing
5. Data Analysis
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