Amplicon sequencing is a targeted sequencing technique used to analyze genetic variation in specific regions of the genome. AmpX, our express amplicon sequencing service, delivers fast and accurate sequencing data that can immediately be used for various applications, including CRISPR validation, 16S metagenomics, antibody sequencing, and many more.
AMPSEQ ADVANTAGE
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Prices starting at $35/sample
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Results in as fast as 3 days
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Products of 150-600bp accepted
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No minimum sample number required
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Free shipping and local pick-up available
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Provides 50K reads per sample
AmpX WORKFLOW
1. Sample Submission
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2. Library Preparation
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4. Data Analysis
3. Sequencing
SAMPLE SUBMISSION GUIDELINES
Sample Type: Purified PCR Product
Amplicon Size: 150-600 bp
Minimum Volume: 20 μL
Recommended Concentration: 15-50 ng/μL
Illumina Adapter Sequences:
For the forward sequencing read: 5’-TCCCTACACGACGCTCTTCCGATCTnnnnnn-3’
For the reverse sequencing read: 5’-GTTCAGACGTGTGCTCTTCCGATCTnnnnnn-3’
* The Ns are optional random nucleotides added to increase sequence diversity.
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COMMON APPLICATIONS
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Antibody Discovery
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16S Metagenomics
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CRISPR Screening
and Validation
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Evolutionary Biology
& Biodiversity Analysis
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